Immunomodulatory Effects of Dioscoreae Rhizome Against Inflammation through Suppressed Production of Cytokines Via Inhibition of the NF-kappaB Pathway
Immune Network
;
: 181-188, 2012.
Article
in English
| WPRIM
| ID: wpr-226027
ABSTRACT
Dioscoreae Rhizome (DR) has been used in traditional medicine to treat numerous diseases and is reported to have anti-diabetes and anti-tumor activities. To identify a bioactive traditional medicine with anti-inflammatory activity of a water extract of DR (EDR), we determined the mRNA and protein levels of proinflammatory cytokines in macrophages through RT-PCR and western blot analysis and performed a FACS analysis for measuring surface molecules. EDR dose-dependently decreased the production of NO and pro-inflammatory cytokines such as IL-1beta, IL-6, TNF-alpha, and PGE2, as well as mRNA levels of iNOS, COX-2, and pro-inflammatory cytokines, as determined by western blot and RT-PCR analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 was also reduced by EDR. Furthermore, activation of the nuclear transcription factor, NF-kappaB, but not that of IL-4 and IL-10, in macrophages was inhibited by EDR. These results show that EDR decreased pro-inflammatory cytokines via inhibition of NF-kappaB-dependent inflammatory protein level, suggesting that EDR could be a useful immunomodulatory agent for treating immunological diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
RNA, Messenger
/
Water
/
Dinoprostone
/
Blotting, Western
/
Cytokines
/
NF-kappa B
/
Interleukin-4
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
Type of study:
Prognostic study
Language:
English
Journal:
Immune Network
Year:
2012
Type:
Article
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