Der f 2 activates phospholipase D in human T lymphocytes from Dermatophagoides farinae specific allergic individuals: Involvement of protein kinase C-alpha
Experimental & Molecular Medicine
;
: 486-492, 2004.
Article
in English
| WPRIM
| ID: wpr-226072
ABSTRACT
The major house-dust mite allergen, Der f 2, stimulates the phospholipase D (PLD) in T lymphocytes from Dermatophagoides farinae specific allergic individuals. PLD activity increased more than two-fold in T cells from allergic patients compared with those cells from normal controls with maximal responses within 30 min after exposure of Der f 2. A well-known PLD activator PKC-alpha was found to be translocated to membrane from cytosol in Der f 2-treated T cells from Dermatophagoides farinae specific allergic individuals. Down-regulation of PKC-alpha with phorbol myristate acetate pretreatment for 24 h abolished Der f 2-induced PLD activation. Ro 320432, PKC inhibitor also reduced the effects of Der f 2-induced PLD activation suggesting that PKC-alpha acts as upstream activator of PLD in Der f 2-treated T cells. Taken together, the present data suggest that Der f 2 can stimulate PLD activity through the PKC-alpha activation in T cells from Dermatophagoides farinae allergic individuals
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phospholipase D
/
Protein Kinase C
/
Skin Tests
/
Tetradecanoylphorbol Acetate
/
T-Lymphocytes
/
Up-Regulation
/
Dermatophagoides farinae
/
Antigens, Dermatophagoides
/
Hypersensitivity, Immediate
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2004
Type:
Article
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