Preferential Recognition of Hydroxyl Radical-Modified Superoxide Dismutase by Circulating Autoantibodies in Patients with Alopecia Areata
Annals of Dermatology
;
: 576-583, 2014.
Article
in English
| WPRIM
| ID: wpr-226137
ABSTRACT
BACKGROUND:
Alopecia areata (AA) is a common form of localized, non-scarring hair loss. The cause of AA is unknown but reports suggest an autoimmune etiology, where oxygen free radicals play an important role.OBJECTIVE:
The aim of this study was to investigate the role of a hydroxyl radicals (.OH)-modified antioxidant enzyme, superoxide dismutase (SOD), in AA autoimmunity.METHODS:
SOD was modified by .OH radicals. Binding characteristics of autoantibodies in AA patients (n=26) against .OH-modified SOD (.OH-SOD) were evaluated by immunoassays and the results were compared with those of healthy, age-matched controls (n=30). The effects of .OH radicals on immunoglobulin G (IgG) isolated from AA patients were studied.RESULTS:
Highly specific binding to .OH-SOD was observed in 32% of the samples of patient sera, whereas normal human sera showed negligible binding with either antigen. Competitive inhibition immunoassays reiterated the results from direct binding. Protein-A-purified IgG from AA patients (AA-IgG) also showed strong binding to .OH-SOD as compared to IgG from normal human controls (p<0.001). In addition, AA-IgG from patients with alopecia universalis recognized .OH-SOD to a greater extent than did AA-IgG from patients with the patchy, persistent type of alopecia. Furthermore, sera from AA patients had lower levels of SOD activity as compared to control sera.CONCLUSION:
This is the first report showing an association between .OH-modified SOD and AA. These novel results demonstrate that .OH radical-mediated changes in SOD present unique neo-epitopes that might contribute to antigen-driven antibody induction in AA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Autoantibodies
/
Superoxide Dismutase
/
Immunoglobulin G
/
Immunoassay
/
Autoimmunity
/
Reactive Oxygen Species
/
Alopecia
/
Alopecia Areata
/
Free Radicals
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2014
Type:
Article
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