Neuroprotective Effects of Growth Hormone Against Hypoxic-Ischemic Brain Injury in Neonatal Rats: 1H Magnetic Resonance Spectroscopic Study
Journal of Korean Medical Science
;
: 122-126, 2007.
Article
in English
| WPRIM
| ID: wpr-226393
ABSTRACT
Using 1H-MRS, we evaluated the effects of growth hormone (GH) as a caspase inhibitor on hypoxic-ischemic injury in neonatal rat brains. The right common carotid arteries of rats were ligated, allowed to recover for 3 hr, and exposed to 8% oxygen for 2 hr. GH was given just prior to HI insult and animals were divided into four groups control, intracerebroventricular (ICV), intracerebroventricular/intraperitoneal (ICV/IP), and intraperitoneal (IP). Localized in vivo 1H-MRS and TUNEL staining were performed 24 hr after HI injury. Lipid/N-acetyl aspartate (NAA) and lipid/creatine (Cr) ratios were used as apoptotic markers. Gross morphologic changes at 2 weeks were used to evaluate the effects of GH. The lipid/NAA ratio was lower in the ICV and ICV/IP groups than in the control, and the lipid/Cr ratio was lower in the ICV group than in the control. The number of TUNEL positive cells was decreased in the ICV and ICV/IP groups, and the degree of morphologic change indicative of brain injury was lower in the ICV group and somewhat lower in the ICV/IP group. The degree of morphologic change correlated with the lipid/NAA and lipid/Cr ratios. These findings suggest that GH exerts neuroprotective effects in cerebral hypoxicischemic injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Growth Hormone
/
Magnetic Resonance Spectroscopy
/
Rats, Sprague-Dawley
/
Aspartic Acid
/
Neuroprotective Agents
/
Creatinine
/
Hypoxia-Ischemia, Brain
/
Lipids
/
Animals, Newborn
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2007
Type:
Article
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