Effects of Halothane, Enflurane or Isoflurane on the m1 or m3 Muscarinic Receptor Expressed in Xenopus Oocytes / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 854-859, 2000.
Article
in Korean
| WPRIM
| ID: wpr-226571
ABSTRACT
BACKGROUND:
Muscarinic receptors are distributed abundantly in the central nervous system and peripheral visceral organs, and have a close relationship with anesthesia. We investigated the effects of halothane, enflurane or isoflurane on m1 or m3 muscarinic signaling.METHODS:
Using two electrode voltage clamps, Ca2+ -activated Cl- currents (ICl(Ca)) were measured in Xenopus oocytes injected with an m1 or m3 receptor mRNA. ICl(Ca) was induced with the application of acetyl beta-methylcholine with or without exposure to volatile anesthetics.RESULTS:
Halothane depressed the m1 and m3 receptor function significantly (m1; 0.49 +/- 0.17 microampere -> 0.1 +/- 0.05 microampere, m3; 0.74 +/- 0.2 -> 0.23 +/- 0.06 microampere, P 0.15 +/- 0.04 microampere, m3; 0.95 +/- 0.34 -> 0.19 +/- 0.05 microampere, P 0.3 +/- 0.09 microampere, P 0.5). From a concentration-response curve, enflurane decreased m1 and m3 signaling dose-dependently.CONCLUSIONS:
Our data suggests that m1 and m3 muscarinic receptors were depressed by halothane, enflurane or isoglurane except for the fact that the m1 receptor was not affected by isoflurane.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Xenopus
/
RNA, Messenger
/
Central Nervous System
/
Receptors, Muscarinic
/
Electrodes
/
Enflurane
/
Halothane
/
Isoflurane
/
Anesthesia
Language:
Korean
Journal:
Korean Journal of Anesthesiology
Year:
2000
Type:
Article
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