Elevated expression of DNMT1 is associated with increased expansion and proliferation of hematopoietic stem cells co-cultured with human MSCs
Blood Research
;
: 25-30, 2017.
Article
in English
| WPRIM
| ID: wpr-226886
ABSTRACT
BACKGROUND:
Mesenchymal stem cells (MSCs) play an important role in hematopoietic stem cell (HSC) maintenance, proliferation, and apoptosis. DNA methyltransferase 1 (DNMT1) is considered an essential factor in the maintenance of HSCs in mammalian cells. Therefore, this study was conducted to evaluate the mRNA expression level of DNMT1 during cord blood (CB)-HSC ex vivo expansion with MSCs.METHODS:
Ex vivo cultures of CB-HSCs were performed in three culture conditions for 7 days cytokines, cytokines with MSCs, and only MSCs. Total and viable cell numbers were counted after 5 and 7 days using trypan blue stain, and the stem cell percentage was then evaluated by flow cytometry. Moreover, in vitro colony-forming unit assay was carried out to detect clonogenic potential of HSCs at days 0 and 7 using MethoCult H4434. Finally, DNMT1 mRNA expression level was evaluated by real-time polymerase chain reaction.RESULTS:
Maximum CB-CD34⁺ cell expansion was observed on day 7 in all the three cultures. After 7 days, ex vivo expansion of CB-CD34⁺ cells indicated a significant decrease in DNMT1 expression in the cytokine cultures, whereas in the two co-culture conditions DNMT1 expression was increased. A significant difference between the number of CD34⁺ and CD34⁻ cells in the cytokine co-culture system was observed.CONCLUSION:
These data indicated that an elevated expression of DNMT1 is associated with increased expansion and proliferation of HSCs co-cultured with human MSCs. Hence, DNMT1 may be a potential factor in the maintenance of expanded HSCs co-cultured with human MSCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Trypan Blue
/
In Vitro Techniques
/
DNA
/
RNA, Messenger
/
Hematopoietic Stem Cells
/
Cell Count
/
Cytokines
/
Apoptosis
/
Coculture Techniques
Limits:
Humans
Language:
English
Journal:
Blood Research
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS