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Identification of Gemella species by 16S ribosomal RNA gene sequencing from two patients with infective endocarditis / 대한내과학회지
Korean Journal of Medicine ; : 591-596, 2006.
Article in Korean | WPRIM | ID: wpr-227049
ABSTRACT
Gemella species are opportunistic pathogens, which cause endocarditis, meningitis, musculoskeletal infections, or pulmonary infections. It is difficult to identify all strains of these species by a conventional culture system. Because 16S rRNA gene has the interspecific polymorphisms to differentiate the bacterial species, 16S rRNA gene sequence analysis could be used to identify these pathogens. To determine the molecular types of two unidentified Gram-positive cocci from two patients with infective endocarditis, PCR amplification and DNA sequencing of the 16S rRNA gene were performed. These sequences of the PCR product were compared with known 16S rRNA gene sequences using GenBank BLAST search. The 16S rRNA gene sequences of two isolates showed > 99% nucleotide similarities with those of Gemella morbillorum (GenBank accession number L14327). Phylogenetic analysis also indicated the close relatedness between these isolates and G. morbillorum.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Ribosomal, 16S / Polymerase Chain Reaction / Gram-Positive Cocci / Sequence Analysis, DNA / Sequence Analysis / Genes, rRNA / Databases, Nucleic Acid / Endocarditis / Gemella / Meningitis Type of study: Diagnostic study Limits: Humans Language: Korean Journal: Korean Journal of Medicine Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Ribosomal, 16S / Polymerase Chain Reaction / Gram-Positive Cocci / Sequence Analysis, DNA / Sequence Analysis / Genes, rRNA / Databases, Nucleic Acid / Endocarditis / Gemella / Meningitis Type of study: Diagnostic study Limits: Humans Language: Korean Journal: Korean Journal of Medicine Year: 2006 Type: Article