Role of annexin-I in cervical cancer cell proliferation / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2199-2204, 1999.
Article
in Korean
| WPRIM
| ID: wpr-227086
ABSTRACT
OBJECTIVES:
To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells.Methods:
By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry.RESULTS:
In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and beta- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells.CONCLUSIONS:
The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tamoxifen
/
Tretinoin
/
HeLa Cells
/
Cell Line
/
Uterine Cervical Neoplasms
/
Blotting, Western
/
Cytoplasm
/
Cell Proliferation
/
Coloring Agents
/
Estradiol
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS