Focal Cerebral Ischemia Reduces Protein Phosphatase 2A Subunit B Expression in Brain Tissue and HT22 Cells / 한국실험동물학회지
Laboratory Animal Research
;
: 73-76, 2011.
Article
in English
| WPRIM
| ID: wpr-227289
ABSTRACT
Protein phosphatase 2A (PP2A) is a serine and threonine protein phosphatase that regulates cell cycle progression and apoptosis. PP2A is composed of various subunits. Among these subunits, subunit B plays an important role in the modulation of PP2A function in the brain. This study investigated PP2A subunit B expression levels after neuronal cell injury. Middle cerebral artery occlusions (MCAO) were surgically induced in adult male rats to induce focal cerebral ischemic injury, and brain tissues were collected 24 h after MCAO. A proteomic approach revealed reduction of PP2A subunit B protein spots in MCAO-operated animals in comparison to sham-operated animals. Western blot analysis confirmed that MCAO induces reductions in PP2A subunit B levels. Moreover, glutamate exposure induces neuronal cell death and leads to reductions of PP2A subunit B levels in a hippocampal-derived cell line. This study demonstrated the decrease of PP2A subunit B in ischemic neuronal cell injury. These results suggest that the decrease of PP2A subunit B after ischemic brain injury can mediate neuronal cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Serine
/
Threonine
/
Brain
/
Brain Injuries
/
Cell Cycle
/
Cell Line
/
Brain Ischemia
/
Blotting, Western
/
Cell Death
/
Apoptosis
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Laboratory Animal Research
Year:
2011
Type:
Article
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