Determination of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector / 한국실험동물학회지
Laboratory Animal Research
;
: 37-40, 2011.
Article
in English
| WPRIM
| ID: wpr-227296
ABSTRACT
This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C18, 4.6x250 mm, 5 microm) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (6040, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acid in acetonitrile for commercial milk, dehydration by adding sodium sulfate to the liquid analytical sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 microm syringe filter. This method had good selectivity and recovery (70.70+/-7.90-110.79+/-14.95%) from the matrices. The LOQs ranged from 9.7 to 9.8 microg/kg for beef and commercial milk. The recoveries met the standards set by the CODEX guideline.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxyclozanide
/
Acetonitriles
/
Phosphoric Acids
/
Sodium
/
Sulfates
/
Syringes
/
Chromatography, High Pressure Liquid
/
Chromatography, Liquid
/
Dehydration
/
Rivers
Language:
English
Journal:
Laboratory Animal Research
Year:
2011
Type:
Article
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