Effect of Anti-vascular Endothelial Growth Factor Antibody on the Survival of Cultured Retinal Ganglion Cells
Korean Journal of Ophthalmology
;
: 360-365, 2017.
Article
in English
| WPRIM
| ID: wpr-227371
ABSTRACT
PURPOSE:
To investigate the effects of anti-vascular endothelial growth factor (VEGF) antibody on the survival of retinal ganglion cell (RGC)-5 cells differentiated with staurosporine under oxidative stress.METHODS:
We used real-time polymerase chain reaction and Western blot to confirm the expression of VEGF, VEGF receptor (VEGFR)-1 and VEGFR-2 in RGC-5 cells differentiated with staurosporine for 6 hours. The differentiated RGC-5 cells were treated with 800 µM hydrogen peroxide (H₂O₂) for 24 hours to induce oxidative stress. Then, the survival rate of RGC-5 was confirmed by lactate dehydrogenase assay at each concentration (0, 0.01, 0.1, and 1 mg) using bevacizumab as the anti-VEGF antibody. The expression of VEGF, VEGFR-1, and VEGFR-2 was confirmed using real-time polymerase chain reaction.RESULTS:
VEGF, VEGFR-1, and VEGFR-2 were all expressed in differentiated RGC-5 cells. When RGC-5 cells were simultaneously treated with bevacizumab and 800 µM H₂O₂, survival of RGC-5 decreased with bevacizumab concentration. VEGF expression in RGC-5 cells increased with increasing concentration of bevacizumab. Similar patterns were observed for VEGFR-1 and VEGFR-2, but the degree of increase was smaller than that for VEGF.CONCLUSIONS:
When bevacizumab was administered to differentiated RGC-5 cells, the cell damage caused by oxidative stress increased. Therefore, given these in vitro study results, caution should be exercised with bevacizumab treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retinal Ganglion Cells
/
Retinaldehyde
/
In Vitro Techniques
/
Blotting, Western
/
Survival Rate
/
Endothelial Growth Factors
/
Oxidative Stress
/
Staurosporine
/
Receptors, Vascular Endothelial Growth Factor
/
Vascular Endothelial Growth Factor Receptor-1
Language:
English
Journal:
Korean Journal of Ophthalmology
Year:
2017
Type:
Article
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