The Cell Cycle Regulatory Effects of High Dose 5-fluorouracil on Breast Cancer Cell Line
Immune Network
;
: 60-64, 2002.
Article
in Korean
| WPRIM
| ID: wpr-228524
ABSTRACT
BACKGROUND:
Chemotherapy with 5-fluorouracil (5-FU) has been one of the mainstay in breast cancer treatment. The effects of high dose 5-FU on cell cycle regulation were studied in breast caner cells.METHODS:
A breast cancer cell line MCF-7 was used. Protein expressions of G1/S cyclins, p21(Waf1/Cip1), cdk2, E2F1 and retinoblastoma were tested by western blot analysis. Immunoprecipitation and immune complex kinase assay were done for the assessment of E2F1/RB interacton and the activity of cdk2 respectively.RESULTS:
p21(Waf1/Cip1) expression was barely detectable in control cells. With addition of 5-FU level of p21(Waf1/Cip1) were induced and cyclin D3 level was decreased as cell growth decreases. In accordance with increased expression of p21(Waf1/Cip1), cyclin E-associated cdk2 kinase activity was reduced. Retinoblastoma protein (RB) became dephosphorylated and E2F-1 binding activity with RB was increased.CONCLUSION:
In this situation of high concentration of 5-FU breast cancer cells tend to be G1/S cell cycle arrested. Overexpression of p21(Waf1/Cip1) and dephosphorylation of RB may mediate the effectss of 5-FU by inhibiting E2F-1 activity, which contributes to G1/S cell cycle arrest. These results could be an indicating landmark for further study of high dose chemotherapy with 5-FU.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphotransferases
/
Retinoblastoma
/
Breast
/
Breast Neoplasms
/
Cell Cycle
/
Cell Line
/
Blotting, Western
/
Retinoblastoma Protein
/
Cyclins
/
Immunoprecipitation
Language:
Korean
Journal:
Immune Network
Year:
2002
Type:
Article
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