Effect of ovarian on FSH release and subunit synthesis in rat anterior pituitary cells in culture / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 41-47, 1999.
Article
in Korean
| WPRIM
| ID: wpr-22854
ABSTRACT
OBJECTIVE:
to determine whether ovarian steroids directly regulate FSH release and B subunit synthesis at the pituitary levelMETHODS:
In vitro study. After applying ovarian steroids in cultured rat anterior pituitary cells, we evaluate the amount of relesed FSH and FSH B subunit using dot-blot hybridization.RESULTS:
Estradiol alone or GnRH-induced did not modulate FSH release and B ubunit synthesis. Although progesterone alone did not alter FSH release, GnRH-induced FSH release was significantly augmented by the treatment of progesterone. Progesterone alone stimulated FSH B mRNA level. Significant increase in FSH B mRNA level was also observed by the combined treatment of progesterone and GnRH. The effects of progesterone on GnRH-induced FSH release was significantly reduced by the treatment of progesterone antagonist, RU 486. The treatment with progesterone antagonist, RU 486 also abolished progesterone-induced FSH B subunit mRNA biosynthesis.CONCLUSION:
From these results, it is, therefore, concluded that estradiol does not seem to be a major regulator for FSH synthesis but progesterone may exert its action at the pituitary level for the synthesis and release of FSH.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Progesterone
/
Steroids
/
RNA, Messenger
/
Mifepristone
/
Gonadotropin-Releasing Hormone
/
Estradiol
/
Estrogens
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
1999
Type:
Article
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