Clinical Performance of the Amplified Mycobacterium tuberculosis Direct Test for the Detection of Mycobacterium tuberculosis in Non-respiratory Specimens / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 315-319, 1999.
Article
in Korean
| WPRIM
| ID: wpr-228758
ABSTRACT
BACKGROUND:
The Amplified Mycobacterium tuberculosis Direct Test (AMTDT) has been developed for the direct detection of M. tuberculosis complex in respiratory specimens. Traditional methods for diagnosis of extrapulmonary tuberculosis such as the acid-fast bacilli (AFB) stain have their well-known limitations. We investigated the usefulness of the AMTDT for a wide range of non-respiratory specimens to establish early diagnosis of extrapulmonary tuberculosis.METHODS:
346 specimens (219 urine, 117 pleural fluid, 6 ascitic fluid, 2 lymph node, 1 gastric aspirate, and 1 pus specimens) from 340 patients referred from November 1997 to September 1998 were tested by the AMTDT. The AMTDT results were evaluated by comparing with clinical diagnosis and smear results.RESULTS:
The overall sensitivity, specificity, and positive and negative predictive values of the AMTDT were 82.9%, 93.8%, 64.2%, and 97.6%, respectively. There were no difference in sensitivity and specificity between pleural fluid and urine specimens. In 31 specimens from tuberculosis patients concurrently tested with AMTDT and stain, 15 were only AMTDT positive and 4 were only stain positive. Among the results considered to be false positive, 47.2% of cases were shown as being less than 150,000 relative light units (RLU). In 30 specimens from tuberculosis patients during or after treatment, all six of the patients with reactivation or aggravation were AMTDT positive, and one case was considered to be false positive.CONCLUSIONS:
Our study demonstrates the efficacy of the AMTDT in diagnosing extrapulmonary tuberculosis. Prudent interpretation of the AMTDT's results is recommended in case of that being less than 150,000 RLU.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Suppuration
/
Tuberculosis
/
Ascitic Fluid
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Early Diagnosis
/
Diagnosis
/
Lymph Nodes
/
Mycobacterium
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
1999
Type:
Article
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