The Polyphenol Chlorogenic Acid Attenuates UVB-mediated Oxidative Stress in Human HaCaT Keratinocytes
Biomolecules & Therapeutics
;
: 136-142, 2014.
Article
in English
| WPRIM
| ID: wpr-228914
ABSTRACT
We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVB exposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280-320 nm). UVB exposure resulted in damage to cellular DNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage and increased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposed cells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levels of the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stress induced by UVB radiation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA Damage
/
DNA
/
Keratinocytes
/
Cell-Free System
/
Cell Survival
/
Chlorogenic Acid
/
Reactive Oxygen Species
/
Apoptosis
/
Superoxides
/
Oxidative Stress
Limits:
Humans
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2014
Type:
Article
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