Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures
The Korean Journal of Parasitology
;
: 613-619, 2014.
Article
in English
| WPRIM
| ID: wpr-229076
ABSTRACT
Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3',4'-dihydroxyflavone, 3',4',5,7-tetrahydroxyflavone (luteolin), and 3,3',4',5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Flavonoids
/
Cells, Cultured
/
Neuroglia
/
Rats, Wistar
/
Neospora
/
Immunologic Factors
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2014
Type:
Article
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