The Expression of Human Beta Defensins by Thermal Injury
Journal of Korean Burn Society
;
: 39-44, 2012.
Article
in Korean
| WPRIM
| ID: wpr-229317
ABSTRACT
PURPOSE:
Defensins are important components of innate immune system. These peptides have antimicrobial activity against a wise variety of pathogens that associated with burn wound infection. In particular, human beta-defensins are expressed in normal epidermal region and showed differential expression of some skin disease. We investigated that expression of human beta-defensin by in vitro and ex-vivo by thermal condition.METHODS:
To investigate the expression of human beta-defensins in acute burn condition, we cultured keratinocytes and used to rat's skin at this experiment. After thermal condition, we showed the expression of beta-defensins-2 (hBD-2), -3 (hBD-3), keratins, keratinocyte differentiation and junction protein levels by RT-PCR and immunohistochemistry (IHC).RESULTS:
HBD-2 & involucrin were down-regulated from 1 hr to 8 hrs in mRNA level. But others were not changed in mRNA level. In protein level, hBD-3 was decreased but pan-cytokeratin and beta-catenin were not changed.CONCLUSION:
HBD-2 was down-regulated in thermal injury. Because thermal injury could induce the influence of keratinocyte differentiation and the decrease of skin protection ability. Our results suggested that human beta-defensins plays an important role in protection by several injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Protein Precursors
/
Skin
/
Skin Diseases
/
Wound Infection
/
Burns
/
RNA, Messenger
/
Immunohistochemistry
/
Keratinocytes
/
Defensins
Limits:
Humans
Language:
Korean
Journal:
Journal of Korean Burn Society
Year:
2012
Type:
Article
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