Effects of Substance P on the Cell Proliferation and IL-2 Production of T Lymphocyte / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 805-818, 1997.
Article
in Korean
| WPRIM
| ID: wpr-229359
ABSTRACT
Immune responses of periodontal tissue may be regulated by products of sensory afferent nerve endings such as neuropeptides. Substance P(SP), a tachykinin neuropeptide, has been previously reported to stimulate the activities of T lymphocyte. Therefore, I examined the role of SP in IL-2 production and cell proliferation by using a homogeneous line of T lymphocytes(Jurkat and HuT78). Cell proliferation rate was determined by [3H]-thymidine incorporation test, and IL-2 was quantitated by the growth rate of CD4+ IL-2-dependent T lymphocyte line CTLL-2. SP stimulated cell proliferation of T lymphocytes at the concentration of 10(-12) and 10(-8)M in a biphasic bell-shape dose-dependent manner. However, SP alone did not induce IL-2 release at the concentration range of 10(-6) to 10(-14)M. The upregulation of IL-2 release was observed when 10(-12)M SP was applied together with mitogens such as Con A or PHA+PMA on T cell lines, especially on Jurkat. Con A or PHA+PMA demonstrated to increase the rate of cell proliferation of Jurkat, which had shown to produce much amount of IL-2 indicating that mitogen-induced cell proliferation might be partially influenced by released IL-2. It was concluded that regulatory effects of SP on the immune/inflammatory response could be mediated through the costimulatory upregulation of IL-2 production and increase of cell proliferation of T lymphocyte.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Neuropeptides
/
Lymphocytes
/
T-Lymphocytes
/
Tachykinins
/
Substance P
/
Up-Regulation
/
Cell Line
/
Interleukin-2
/
Cell Proliferation
/
Mitogens
Language:
Korean
Journal:
The Journal of the Korean Academy of Periodontology
Year:
1997
Type:
Article
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