Inhibitory effects of saponins from Anemarrhena asphodeloides Bunge on the growth of vascular smooth muscle cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 185-191, 2006.
Article
in English
| WPRIM
| ID: wpr-229704
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of saponins from Anemarrhena asphodeloides Bunge (SAaB) (Botanical Name Anemarrhena Asphodeloidis Rhizoma) on the growth of vascular smooth muscle cells (VSMCs).</p><p><b>METHODS</b>Cell proliferation was measured by a newly developed cell proliferation reagent, WST-1. Cell apoptosis was assayed by flow cytometry through detecting annexin V. Nitric oxide production was evaluated using confocal laser scanning microscopy with diaminofluorescein diacetate (DAF-2, DA). Cell aldose reductase (AR) activity, as well as the effect of Epalrestat and interleukin-1beta were also explored.</p><p><b>RESULTS</b>WST assay showed that cell proliferation induced by serum was significantly inhibited by SAaB (P<0.01). Flow cytometry analysis revealed that SAaB could enhance apoptotic rate of VSMCs (P<0.01). Nitric oxide production was significantly enhanced after administration of SAaB and interleukin-1beta. Moreover, AR activity of VSMCs was also remarkably inhibited by both SAaB and Epalrestat (P<0.01).</p><p><b>CONCLUSION</b>SAaB can inhibit proliferation and enhance apoptosis of VSMCs. It may protect vascular cells by inhibiting VSMC proliferation and augmenting apoptotic rate of VSMCs via NO-dependent pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Saponins
/
Cells, Cultured
/
Chemistry
/
Rats, Sprague-Dawley
/
Annexin A5
/
Microscopy, Confocal
/
Fluorescein
/
Cell Biology
/
Anemarrhena
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2006
Type:
Article
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