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Protective effect of isoflurane and sevoflurane on ischemic neurons and expression of Bcl-2 and ICE genes in rat brain / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 143-146, 2006.
Article in English | WPRIM | ID: wpr-229712
ABSTRACT
<p><b>OBJECTIVE</b>To study the protective effect of volatile anesthetics, isoflurane and sevoflurane, on ischemic neurons after cerebral ischemia-reperfusion in rats and its possible molecular mechanism.</p><p><b>METHODS</b>Rat cerebral ischemia-reperfusion model was developed by occlusion of the middle cerebral artery (MCA) and bilateral common carotid arteries (CCAs) 1 h after reperfusion. Using flow cytometry (FCM) and Northern blot hybridization, we calculated the number of apoptotic bodies and detected the expression of bcl-2 mRNA and interleukin-1beta converting enzyme (ICE) mRNA.</p><p><b>RESULTS</b>The apoptotic bodies in hippocampus analyzed by FCM peaked at appeared 24 h after reperfusion, and decreased about 54% and 40%, respectively, after treatment with isoflurane and sevoflurane, as compared with ischemic group. There was no significant difference in the expression of bcl-2 mRNA and ICE mRNA between the inhaled anesthetic groups and ischemic group in hippocampus 24 h after MCA/CCAs occlusion.</p><p><b>CONCLUSION</b>Isoflurane and sevoflurane partially inhibit apoptosis but have no significant effect on the expression of bcl-2 and ICE genes.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Reperfusion / Brain Ischemia / Rats, Wistar / Apoptosis / Anesthetics, Inhalation / Proto-Oncogene Proteins c-bcl-2 / Caspase 1 / Drug Therapy Limits: Animals Language: English Journal: Biomedical and Environmental Sciences Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Reperfusion / Brain Ischemia / Rats, Wistar / Apoptosis / Anesthetics, Inhalation / Proto-Oncogene Proteins c-bcl-2 / Caspase 1 / Drug Therapy Limits: Animals Language: English Journal: Biomedical and Environmental Sciences Year: 2006 Type: Article