Protective effect of isoflurane and sevoflurane on ischemic neurons and expression of Bcl-2 and ICE genes in rat brain / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 143-146, 2006.
Article
in English
| WPRIM
| ID: wpr-229712
ABSTRACT
<p><b>OBJECTIVE</b>To study the protective effect of volatile anesthetics, isoflurane and sevoflurane, on ischemic neurons after cerebral ischemia-reperfusion in rats and its possible molecular mechanism.</p><p><b>METHODS</b>Rat cerebral ischemia-reperfusion model was developed by occlusion of the middle cerebral artery (MCA) and bilateral common carotid arteries (CCAs) 1 h after reperfusion. Using flow cytometry (FCM) and Northern blot hybridization, we calculated the number of apoptotic bodies and detected the expression of bcl-2 mRNA and interleukin-1beta converting enzyme (ICE) mRNA.</p><p><b>RESULTS</b>The apoptotic bodies in hippocampus analyzed by FCM peaked at appeared 24 h after reperfusion, and decreased about 54% and 40%, respectively, after treatment with isoflurane and sevoflurane, as compared with ischemic group. There was no significant difference in the expression of bcl-2 mRNA and ICE mRNA between the inhaled anesthetic groups and ischemic group in hippocampus 24 h after MCA/CCAs occlusion.</p><p><b>CONCLUSION</b>Isoflurane and sevoflurane partially inhibit apoptosis but have no significant effect on the expression of bcl-2 and ICE genes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Reperfusion
/
Brain Ischemia
/
Rats, Wistar
/
Apoptosis
/
Anesthetics, Inhalation
/
Proto-Oncogene Proteins c-bcl-2
/
Caspase 1
/
Drug Therapy
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2006
Type:
Article
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