Inhibitory effects of alpha-zearalenol on angiotensin II-induced integrin beta3 mRNA via suppression of nuclear factor-kappaB / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 314-320, 2005.
Article
in English
| WPRIM
| ID: wpr-229749
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of alpha-zearalenol on angiotensin II-induced beta3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs).</p><p><b>METHODS</b>The mRNA level in integrin beta3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-kappaB activity was determined by the luciferase activity assay of plasmid NF-kappaB-LUC.</p><p><b>RESULTS</b>The angiotensin II-induced beta3 integrin mRNA expression was inhibited by alpha-zearalenol and 17beta-estradiol (10 nmol/L -1 micromol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nomega-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17beta-estradiol suppressed the angiotensin II-induced activation of NF-kappaB in endothelial cells.</p><p><b>CONCLUSION</b>Alpha-zearalenol inhibits angiotensin II-induced integrin beta3 mRNA expression by suppressing NF-kappaB activation in endothelial cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Zeranol
/
RNA, Messenger
/
Angiotensin II
/
Endothelium, Vascular
/
Receptors, Estrogen
/
Cells, Cultured
/
Gene Expression Regulation
/
NF-kappa B
Limits:
Female
/
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2005
Type:
Article
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