The cytogenetic features of Burkitt leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 214-217, 2008.
Article
in Zh
| WPRIM
| ID: wpr-229787
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the incidence of chromosomal abnormalities in Burkitt leukemia (BL).</p><p><b>METHODS</b>Conventional cytogenetics (CC) was carried out to detect the karyotypes. Meanwhile, three color interphase fluorescence in situ hybridization (FISH) was used to detect the t(8;14)(q24;q32) in 17 newly diagnosed BL.</p><p><b>RESULTS</b>The results showed that the incidence of chromosomal abnormalities was 41.2% (7/17) by CC technique, including one of each for simple t(8;14)(q24;q32), complex chromosomal abnormality containing t(8;14)(q24;q32), t(8;22)(q24;q11), the complex chromosomal abnormality of t(12;22)(?;?), t(2;13)(?;?) with + 12, and one with two marker chromosomes. FISH method detected eight cases of t(8;14)(q24;q32), including the two detected by CC technique. Five samples (5/8) showed 2A1G1O2F (two blue, one green, one orange and two yellow signals in interphase nuclei), while three samples (3/8) showed 2A1G1O1F(two blue, one green, one orange and one yellow signals in interphase nuclei).</p><p><b>CONCLUSION</b>Two different breakpoints have been identified on the c-Myc locus on 8q24. Interphase FISH was more sensitive in detecting t(8;14)(q24;q32), and it is an important complement to CC. It should be used as a routine method for diagnosis of BL.</p>
Full text:
1
Index:
WPRIM
Main subject:
Chromosome Aberrations
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Burkitt Lymphoma
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In Situ Hybridization, Fluorescence
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Genetics
Limits:
Adolescent
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Adult
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2008
Type:
Article