Detection of Y chromosome microdeletions in AZF region by liquid chip technology / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 86-88, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-229813
ABSTRACT
<p><b>OBJECTIVE</b>To establish a liquid chip technology to detect Y chromosome microdeletions in Chinese infertile males with azoospermia or oligozoospermia.</p><p><b>METHODS</b>Multiplex PCR and liquid chip technology were used to detect the Y chromosome microdeletions in AZF region in 178 infertile patients with azoospermia and 134 infertile patients with oligozoospermia as well as 40 fertile control men.</p><p><b>RESULTS</b>Forty out of 312 patients (12.8%) were found to have deletions in AZF region. The microdeletion frequency was 14%(25/178) in the azoospermic group, 9.6%(11/114) in the oligospermic and 20%(4/20) in the severe oligospermic group.</p><p><b>CONCLUSION</b>The authors developed a high-throughput, fast and simple assay to screen the AZF region microdeletions of Y chromosome.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligospermia
/
Base Sequence
/
Sequence Tagged Sites
/
Chromosome Deletion
/
Oligonucleotide Array Sequence Analysis
/
Chromosomes, Human, Y
/
Asian People
/
Electrophoresis, Polyacrylamide Gel
/
Azoospermia
/
Genetics
Type of study:
Diagnostic study
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS