Bioluminescent imaging monitoring of a anti-angiogenesis therapeutic gene vasostatin in tumor cell PC3 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 312-317, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-229982
ABSTRACT
<p><b>OBJECTIVE</b>To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.</p><p><b>METHODS</b>We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.</p><p><b>RESULTS</b>We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.</p><p><b>CONCLUSION</b>Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Recombinant Fusion Proteins
/
Genes, Reporter
/
Gene Transfer Techniques
/
Calreticulin
/
Cell Line, Tumor
/
Luciferases, Firefly
/
Genetics
/
Luminescent Measurements
/
Metabolism
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2007
Type:
Article
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