Genotyping of genital human papillomavirus by DNA sequencing and luminex methods / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 181-185, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-230008
ABSTRACT
<p><b>OBJECTIVE</b>To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).</p><p><b>METHOD</b>HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).</p><p><b>RESULT</b>Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.</p><p><b>CONCLUSION</b>A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Virology
/
DNA, Viral
/
Base Sequence
/
Polymerase Chain Reaction
/
Oligonucleotide Array Sequence Analysis
/
Papillomavirus Infections
/
Female Urogenital Diseases
/
Genetics
/
Genotype
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2007
Type:
Article
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