Effects of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of HL-60 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 458-461, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-230245
ABSTRACT
This study was aimed to investigate the effect of chemokine-like factor superfamily 8 (CKLFSF8) on proliferation and expression of epidermal growth factor receptor (EGFR) of HL-60 cells. Expression of CKLFSF8 mRNA on HL-60 cell line was assayed by RT-PCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL-60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed in HL-60 cells. After transfection, cell proliferation was inhibited (P < 0.05) and the expression of EGFR in HL-60 cells was also discovered to be inhibited (P < 0.05). It is concluded that the proliferation and expression of EGFR in HL-60 cells can be inhibited by transfection of CKLFSF8. The novel chemokine may provide a new approach in the treatment of leukemia.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
HL-60 Cells
/
Chemokines
/
Cell Proliferation
/
MARVEL Domain-Containing Proteins
/
ErbB Receptors
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
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