Expression and role of toll-like receptors in U937 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 449-453, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-230247
ABSTRACT
The aim of study was to explore the potential application of targeting at Toll-like receptors (TLRs) in the immunotherapy of acute myelocytic leukemia, and to investigate the expression of TLR and the effects of TLR 8 agonist ssRNA40/LyoVec on proliferation, apoptosis and cell cycle of U937 cells. The expression of TLR 1 - 9 in U937 cells was detected by using reverse transcription polymerase chain reaction (RT-PCR) and the expression of TLR 8 was assayed by flow cytometry (FCM). The effect of TLR 8 agonist, ssRNA40/LyoVec, at different concentrations on U937 cells proliferation was evaluated by CCK-8, apoptosis and cell cycle were detected by FCM. The results showed that U937 cells expressed TLR 1 - 9. TLR 8 agonist ssRNA40/LyoVec could inhibit the growth of U937 cells both in time-and dose-dependent manner and the inhibitory rate could reach 70%. It also increased the percentage of cells in G(0)/G(1) phase. There was no significant difference in percentage of apoptotic cells between control and treated groups. It is concluded that TLRs including TLR 1 - 9 express on U937 cells and TLR 8 agonist ssRNA40/LyoVec may be able to inhibit the growth of U937 cells, arrest the cells in G(0)/G(1) phase, but have no effect of promoting apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Leukemia, Myeloid, Acute
/
Cell Cycle
/
Apoptosis
/
U937 Cells
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Proliferation
/
Dose-Response Relationship, Drug
/
Toll-Like Receptors
/
Toll-Like Receptor 8
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
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