Your browser doesn't support javascript.
loading
Detection of p15 gene methylation or deletion status in different malignant cell lines by using hemi-nested methylation specific polymerase chain reaction / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 382-386, 2007.
Article in Chinese | WPRIM | ID: wpr-230263
ABSTRACT
This study was aimed to investigate the methylation or deletion status of p15 gene in different malignant cell lines, and further to clarify their roles in the development and progression of malignant tumors. Hemi-nested methylation specific polymerase chain reaction (hn-MSP) was adopted to analyze p15 gene methylation or deletion status in 20 malignant tumor cell lines and mononuclear cells or normal cell lines in healthy people, as well as to evaluate its sensitivity and specificity. The results showed that among all of the cell lines, Molt-4, KG1, NCE, Raji, SMMC-7221, CA46, SW480 and NCI-H446 were partial methylated with CDKN2B gene, and its sensitivity of detection of p15 gene methylation was up to 1.0 x 10(-5), also it had great specificity. Peripheral blood mononuclear cell (MNCs) from healthy volunteer, HL-60, HepG2, 293, HeLa, SGC7901, U266 and CEM were unmethylated; and K562, NB4, GMC, Jurkat seemed to have deletion or mutation of p15 gene. It is concluded that the incidence of p15 gene methylation or deletion in many tumours, especially malignant hematopathy, is frequent, they correlate with disease progression and prognosis. Hn-MSP is highly sensitive and specific in analyzing p15 gene methylation, deserving in clinical application.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Tumor Cells, Cultured / Gene Expression Regulation, Neoplastic / Polymerase Chain Reaction / Gene Deletion / CpG Islands / Hematologic Neoplasms / DNA Methylation / Cyclin-Dependent Kinase Inhibitor p15 / Genetics Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2007 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Tumor Cells, Cultured / Gene Expression Regulation, Neoplastic / Polymerase Chain Reaction / Gene Deletion / CpG Islands / Hematologic Neoplasms / DNA Methylation / Cyclin-Dependent Kinase Inhibitor p15 / Genetics Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2007 Type: Article