An evaluation of transforming growth factor-beta 1 in diagnosing hepatocellular carcinoma and metastasis / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 503-508, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-230552
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the dynamic expressions of TGF-beta 1 and TGF-beta 1 mRNA at different stages of hepatocellular carcinoma (HCC) development and their use in clinical diagnosis.</p><p><b>METHODS</b>Hepatoma models were developed with 2-FAA using male Sprague-Dawley (SD) rats. Morphological changes of the rat liver histological preparations (H and E stained) were studied. The fragment of TGF-beta 1 gene obtained was amplified by nested RT-PCR. Dynamic change of TGF-beta 1 level was quantitatively analyzed by ELISA. The distribution of TGF-beta 1 in the cells and its gene expression were detected in human HCC tissues.</p><p><b>RESULTS</b>The progressive increases of hepatic TGF-beta 1 and TGF-beta 1 mRNA were observed in rat hepatocytes which progressed from granular degeneration, atypical hyperplasia and finally to HCC development induced by 2-FAA. The expression levels in HCC tissues were significantly higher than those in the normal and degenerative ones. TGF-beta 1 was shown in rat hepatocytes by immunohistochemistry. Plasma TGF-beta 1 was detected in 89.5% of all the patients with HCC, but it was detected in 93.3% of them who had an AFP less than 400 microg/L. TGF-beta 1 mRNA showed a stronger expression in HCC tissues. TGF-beta 1 mRNA was found in peripheral blood mononuclear cells from all HCC patients with extrahepatic metastasis.</p><p><b>CONCLUSION</b>TGF-beta 1 may participate in hepatocyte canceration. The overexpression of TGF-beta 1 and TGF-beta 1 mRNA could be useful markers for early diagnosis and predicting prognosis of HCC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Prognosis
/
Blood
/
RNA, Messenger
/
Biomarkers, Tumor
/
Rats, Sprague-Dawley
/
Carcinoma, Hepatocellular
/
Diagnosis
/
Transforming Growth Factor beta1
/
Liver Neoplasms, Experimental
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2007
Type:
Article
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