Human amniotic fluid influenced by different sampling methods during 1H NMR-based metabonomics analysis / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 731-735, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-230795
ABSTRACT
To assess the influence of different sampling methods on Human Amniotic Fluid (HAF) during metabonomics analysis, and to establish a metabolite profile database for normal human amniotic fluid, four experimental groups (the group of freeze-drying, of freeze-thawing, of storage at -20 degrees C, and of keeping in room temperature) and a control group were investigated by use of 1H-NMR spectroscopy, respectively; the data of H-NMR spectroscopy was treated by principal components analysis (PCA). The results showed that, by comparison with the control, there were distinct differences in the experimental groups except the group of storage at -20 degrees C. Therefore, It is possible to use 1H-NMR-based metabonomics technique for analysis of HAF; moreover, during the tests, careful treatments of HAF should be institued to minimize the influence on the samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reference Standards
/
Specimen Handling
/
Magnetic Resonance Spectroscopy
/
Principal Component Analysis
/
Metabolome
/
Metabolomics
/
Amniotic Fluid
/
Metabolism
/
Methods
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2010
Type:
Article
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