Cloning and expression analysis of protein kinase SmSnRK2.4 from Salvia miltiorrhiza / 中国中药杂志

ABSTRACT

Sucrose non-fermenting 1-related protein kinase 2(SnRK2) plays a key role in abiotic stress signaling in plants. In this study, we cloned a SmSnRK2.4 gene belonging to subclass I of SnRK2 from Salvia miltiorrhiza by screening its transcriptome database. The SmSnRK2.4 gene contains 8 introns and 9 exons, with a 1 068 bp open reading frame encoding a polypeptide of 355 amino acids, the predicted molecular mass of which is 40.63 kDa. Prokaryotic expression of SmSnRK2.4 protein using pMAL-c2X as the expression vector displayed that the recombinant protein of SmSnRK2.4 gene in E. coli was consistent with the predicted size. A 3 000 bp promoter sequence of SmSnRK2.4 contained some stress-responsive elements and hormone-responsive elements. Quantitative real-time PCR analysis revealed that the expression of SmSnRK2.4 in root was much higher than that in stem and leaf, SmSnRK2.4 was strongly induced by PEG stress, weakly induced by ABA stress. This research provided a basis for further study of the SmSnRK2.4 gene playing the role in accumulate mechanism of secondary metabolites in S. miltiorrhiza under drought.