Cloning,expression and functional identification of secoisolariciresinol dehydrogenase gene from Dysosma versipellis callus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4568-4571, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-231019
ABSTRACT
Secoisolariciresinol dehydrogenase (SDH) is a key enzyme involved in the biosynthetic pathway of podophyllotoxin.In this study, two SDH candidate genes,SO282 and SO1223, were cloned from callus of Dysosma versipellis by homology-based PCR and rapid amplification of cDNA end (RACE).The SDH candidate genes were expressed in Escherichia coli and the subsequent enzyme assay in vitro showed that recombinant SO282 had the SDH activity. These results pave the way to the follow-up investigation of the biosynthetic of podophyllotoxin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2016
Type:
Article
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