Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 412-414, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-231035
ABSTRACT
<p><b>OBJECTIVE</b>To construct a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.</p><p><b>METHODS</b>The SEA gene and a DNA fragment encoding the signal for GPI-anchor attachment of hPLAP -1 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric GPI- anchored SEA molecule with gene splicing by overlap extension. The resulting chimera was cloned in pGEM-T vector and verified by sequencing analysis.</p><p><b>RESULT</b>A chimeric SEA-hPLAP-1 cDNA was successfully constructed with gene splicing by overlap extension.</p><p><b>CONCLUSION</b>Gene splicing by overlap extension is a successful specific PCR technique for gene recombination.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Base Sequence
/
RNA Splicing
/
Polymerase Chain Reaction
/
Alkaline Phosphatase
/
Enterotoxins
/
GPI-Linked Proteins
/
Genetics
/
Isoenzymes
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2003
Type:
Article
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