Increase of LTB4 level and expression of LTA4-hydrolase mRNA in lung tissue and cerebral cortex in asthmatic rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 296-322, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-231063
ABSTRACT
<p><b>OBJECTIVE</b>To investigate antigen-induced changes of leukotriene B(4)(LTB(4))content and LTA(4)-hydrolase mRNA expression in lung tissue and cerebral cortex in sensitized rats.</p><p><b>METHODS</b>The contents of LTB(4) in lung tissue and cerebral cortex homogenates and LTA(4)-hydrolase mRNA expression after antigen challenge by aerosol were respectively detected by reverse-phase high performance liquid chromatography(RP-HPLC) and semi-quantitative RT-PCR.</p><p><b>RESULT</b>The LTB(4) levels in lung tissue and cerebral cortex homogenates in asthmatic rats were significantly higher than those in control (P%0.05), and LTA4-hydrolase mRNA expression was also increased in asthmatic group. Dexamethason(DXM, 0.5 mg/kg, i.p.) decreased the LTB(4) content and inhibited the LTA(4)-hydrolase mRNA expression significantly in asthmatic rats(P%0.05).</p><p><b>CONCLUSION</b>LTB(4) content and LTA(4)-hydrolase mRNA expression in cerebral cortex and lung tissue are increased in asthmatic rats, and there may exist neuroimmunological cross-talking between central nervous system and lung tissue in asthma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
RNA, Messenger
/
Cerebral Cortex
/
Chemistry
/
Rats, Sprague-Dawley
/
Leukotriene B4
/
Epoxide Hydrolases
/
Genetics
/
Lung
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2003
Type:
Article
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