Preparation of monoclonal antibodies against oh(8)dG and their biological characteristics / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 33-36, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-231127
ABSTRACT
<p><b>OBJECTIVE</b>To prepare monoclonal antibodies against oh(8)dG and to evaluate the relationship between Hp infection and oxidative DNA damage by detecting oh8dG in gastric mucosa.</p><p><b>METHODS</b>BALB/C mice were immunized with BSA-oh(8)dG conjugate, monoclonal antibodies were prepared by hybridoma technique, the biological characteristics of antibodies were analysed by competitive ELISA, Western blot and immunohistochemistry.</p><p><b>RESULTS</b>Two strains of hybridoma cell were obtained. ELISA and Western blot indicated that the antibodies were fairly specific for oh(8)dG. In immunohistochemistry,the positive rate of oh(8)dG expression in Hp positive tissues and Hp negative tissues was 55% and 5%, respectively(P<0.01).</p><p><b>CONCLUSION</b>The prepared antibodies can specially recognize oh(8)dG and immunohistochemistry with the monoclonal antibodies showed Hp infection can increase oh(8)dG level in gastric mucosa.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Immunohistochemistry
/
Chemistry
/
Blotting, Western
/
Helicobacter pylori
/
Helicobacter Infections
/
Deoxyguanosine
/
Diagnosis
/
Allergy and Immunology
/
Gastric Mucosa
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS