Autophagy inhibitor 3-MA decreases the production and release of infectious enterovirus 71 particles / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine whether or not enterovirus 71 (enteroviurs 71, EV71) may induce autophagy and affect the production and release of EV71 after the treatment of autophagy inhibitor.</p><p><b>METHODS</b>Western blots were performed to examine the conversion of LC3-I to LC3- II and the degradation of P62 after the RD-A cells were infected with EV71. CCID50 was determined by checking the virus titer in the supernatant of cells that treated with autophagy inhibitor 3-MA.</p><p><b>RESULTS</b>EV71 infection enhances the type conversion of LC3 and degradation of P62. The infectious virus particles were decreased after the treatment of 3-MA.</p><p><b>CONCLUSION</b>EV71 infection could induce cell autophagy and the autophagy might contribute to the production and release of infectious EV71 particles.</p>