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Construction of vectors expressing M2 and NA genes of H5N1 influenza virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 167-169, 2011.
Article in Chinese | WPRIM | ID: wpr-231161
ABSTRACT
<p><b>OBJECTIVE</b>To construct vectors expressing M2 and NA genes of H5N1 influenza virus.</p><p><b>METHODS</b>Based on the human H5N1 avian influenza virus (A/Anhui/1/2005) isolated in china, M2 and NA genes were amplified by PCR. M2 or NA gene was subcloned into pStar vector to construct recombinant pStar-M2/, pStar-/M2, pStar-NA/and pStar-NA/. Furthermore, both of the M2 and NA genes were subcloned into pStar to construct two genes co-expressing recombinant pStar-M2/NA and pStar-NA/M2. Expression of the genes were detected by IFA after transfection of 293 cells with the recombinant plasmids.</p><p><b>RESULTS</b>Recombinant plasmids were constructed and identified by restriction endonuclease digestion. Expression of the genes cloned into the recombinant plasmids was confirmed by IFA.</p><p><b>CONCLUSION</b>Recombinant plasmids expressing M2 and/or NA genes of H5N1 influenza virus were constructed, which provided basis for development of influenza DNA vaccine.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Viral Proteins / Cell Line / Viral Matrix Proteins / Influenza A Virus, H5N1 Subtype / Genetic Vectors / Genetics / Metabolism / Neuraminidase Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Viral Proteins / Cell Line / Viral Matrix Proteins / Influenza A Virus, H5N1 Subtype / Genetic Vectors / Genetics / Metabolism / Neuraminidase Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2011 Type: Article