The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 152-154, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-231164
ABSTRACT
<p><b>OBJECTIVE</b>To establish the real-time fluorescent PCR method for detecting enterovirus, enterovirus 71 and Coxsackievirus A 16 nucleic acid.</p><p><b>METHODS</b>Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.</p><p><b>RESULTS</b>The real-time fluorescent PCR positive rates of EV, EV71 and Cox A16 were 73.7%, 60.5%, 13.2%; the conventional RT-PCR were 71.1%, 55.3%, 13.2%. There were no significant differences between the two methods.</p><p><b>CONCLUSION</b>The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Virology
/
Chemistry
/
Polymerase Chain Reaction
/
DNA Primers
/
Enterovirus A, Human
/
Diagnosis
/
Fluorescent Dyes
/
Genetics
/
Hand, Foot and Mouth Disease
Type of study:
Diagnostic study
/
Etiology study
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Type:
Article
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