Enterovirus 71 induces apoptosis in a Bax dependent manner / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the molecular mechanism of apoptosis induced by enterovirus 71 (EV71) infection.</p><p><b>METHODS</b>The effects of EV71 on Rhabdomyosarcoma (RD) cell viability were detected by CCK8 assay. EV71-induced apoptosis on RD cells were detected by Hoechst 33342 staining and Western blot targeting Caspase 3, 8 and PARP. Bax conformational change was detected by immunoprecipitation with Bax 6A7 antibody.</p><p><b>RESULTS</b>EV71 decreased the viability of RD cells and induces the activation of Caspase 3, 8 and PARP. Bax expression increases in RD cells after EV71 infection, and Bax conformational change also can be detected after EV71 infection.</p><p><b>CONCLUSION</b>Our study reveals that EV71 induces Caspase-dependent apoptosis by Bax conformational change.</p>