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The expression of Aeromonas hydrophila ompTS gene and the immunogenicity of recombinant OmpTS / 生物工程学报
Chinese Journal of Biotechnology ; (12): 300-303, 2002.
Article in Chinese | WPRIM | ID: wpr-231330
ABSTRACT
Primers were designed based on ompTS gene reported recently. With the specific primers, one target fragment about 1024 bp lacking the signal sequence of ompTS gene was amplified from A. hydrophila genomic DNA via PCR. The ompTS gene was hyperexpressed using gene fusion expression vector pRSET system, and the recombinant OMP exhibited a size of 39.9 kD with SDS-PAGE and Western blot analysis, which showed about 51% of total lysate proteins. Antibody to the purified recombinant OMP reacted not only to the recombinant OMP but also to the purified OMPs from A. hydrophila in ELISA and the 36.9 kD OMP in Western blot. The result indicates that the recombinant OMP has the same epitope with the nature one.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Bacterial Outer Membrane Proteins / Recombinant Proteins / Molecular Sequence Data / Base Sequence / Gene Expression / Polymerase Chain Reaction / Amino Acid Sequence / Aeromonas hydrophila / Allergy and Immunology Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2002 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Bacterial Outer Membrane Proteins / Recombinant Proteins / Molecular Sequence Data / Base Sequence / Gene Expression / Polymerase Chain Reaction / Amino Acid Sequence / Aeromonas hydrophila / Allergy and Immunology Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2002 Type: Article