Cloning and expression of spinach glycolate oxidase in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 212-215, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-231348
ABSTRACT
The cDNA coding spinach glycolate oxidase (GO) was amplified by RT-PCR using the total RNA of spinach leaves as the template, and was cloned into cloning vector pMD18-T. After the DNA sequence was determined, the go gene was subcloned into E. coli expression vector pBV220, pET-22b(+), pTIG-Trx and pThioHisC. SDS-PAGE analysis revealed that the recombinant E. coli BL21 (DE3) (pTIG-Trx-GO) and E. coli BL21 (DE3) (pET-22b(+)-GO) expressed the predicted 38 kD glycolate oxidase, and the enzyme activity was also detected.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Gene Expression
/
Cloning, Molecular
/
Spinacia oleracea
/
Alcohol Oxidoreductases
/
Escherichia coli
/
Genetics
/
Isopropyl Thiogalactoside
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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