Expression of adenovirus-mediated pGH cDNA with first intron in CHO cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 193-197, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-231352
ABSTRACT
The recombinant adenoviruses containing pGH cDNA and pGH cDNA with the first intron under the control of CMV promoter were constructed respectively by homogenous recombination method. The results showed that the recombinant adenoviruses could mediate pGH cDNA expression in CHO cells infected with the recombinant adenoviruses. The expression level of pGH cDNA with the first intron increased by 117% compared with pGH cDNA without intron. This indicate that the first intron of pGH gene have the function of improving the expression of the pGH gene.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Recombinant Fusion Proteins
/
Growth Hormone
/
Introns
/
Genetic Engineering
/
Gene Expression
/
Adenoviridae
/
Promoter Regions, Genetic
/
CHO Cells
/
Cytomegalovirus
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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