Effect of trypsin on the rat keratinocyte separation and subculture / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 59-62, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-231372
ABSTRACT
The effect of trypsin on the separation an subculture of the keratinocytes was investigated in this work. It was found that when 0.25% trypsin was employed for 5 minutes to separate keratinocytes, the number of active keratinocytes and the cells capable of forming colony were higher than those of other experimental conditions. The maximum attached ratio of primary keratinocytes was obtained when skin tissues were treated at 0.05% concentration of trypsin. With the increase of the trypsin concentrations, the attached ratio, attachment rate constant, and colony forming efficiency were all increased. Thus, 0.25% concentration of trypsin was recommended for separating and subculturing the keratinocytes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Stem Cells
/
Trypsin
/
Keratinocytes
/
Cell Count
/
Cell Separation
/
Cell Survival
/
Cell Culture Techniques
/
Cell Biology
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Type:
Article
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