Preliminary study of establishing and assessing IBS-D model rats of gan stagnation and Pi deficiency syndrome / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1507-1514, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-231654
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new disease-syndrome-symptom integrated diarrhea-predominant irritable bowel syndrome (IBS-D) rat model of Gan stagnation and Pi deficiency syndrome (GSPDS).</p><p><b>METHODS</b>(1) The model establishment method:
We combined mother-infant separation, chronic restraint, and senna gavage to establish a new IBS-D model of GSPDS. Totally 48 experimental rats were divided into the normal group (Group A), the mother-infant separation group (Group B), the chronic restraint group (Group C), and the senna gavage group (Group D), the mother-infant separation + senna gavage group (Group E), and the mother-infant separation + chronic restraint + senna gavage group (Group F), 8 in each group. (2) The model evaluationmethod:
We used pain threshold indicating colorectal distension to represent for the visceral sensitivity, thus evaluating the establishment of "disease" model; open field test and serum D-xylose levels to evaluate the establishment of GSPDS model; defecation numbers of grain and loose stool rate to evaluate the establishment of diarrhea symptom.</p><p><b>RESULTS</b>(1) Compared with Group A, the body weight gained less in Group F, showing statistical difference (P < 0.05). (2) The pain threshold significantly decreased in Group F, showing statistical difference when compared with Group A (P < 0.05). (3) Compared with Group A, the total cross number, the standing number, and the decoration number in Group F significantly decreased (P < 0.05). (4) Compared with Group A, the serum D-xylose level of Group F significantly decreased (P < 0.05). (5) Compared with Group A, the defecation numbers of grain and the loose stool rate significantly increased, showing statistical difference (P < 0.05).</p><p><b>CONCLUSIONS</b>A new disease-syndrome-symptom integrated IBS-D animal model of GSPDS successfully established might be a better animal model used for studying IBS by Chinese medicine. However, further studies are needed.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rats, Sprague-Dawley
/
Irritable Bowel Syndrome
/
Diagnosis
/
Diarrhea
/
Disease Models, Animal
/
Animals, Newborn
/
Medicine, Chinese Traditional
Type of study:
Diagnostic study
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS