Effect of sanshui baihu decoction on the proliferation of fibroblast-like synoviocytes and its secretion of IL-6 and IL-17 / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1385-1388, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-231677
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Sanshui Baihu Decoction (SBD) containing serum on the proliferation of in vitro cultured fibroblast-like synoviocytes (FLS) derived from rheumatoid arthritis (RA) and osteoarthritis (OA) and its secretion of interleukin-6 (IL-6) and IL-17, and to explore the pharmacological mechanism of SBD.</p><p><b>METHODS</b>The FLS obtained from cultured RA and OA patients' synovial tissue were cultured and passaged in vitro in a routine way. The cultured medium was changed to DMEM with 20% SBD containing serum and cultured for 72 h after cultured for 3 to 6 generations. The proliferation rate of FLS was detected by MTT assay. Levels of IL-6 and IL-17 in the supernatant were detected by ELISA. Leflunomide and saline containing serum were used as positive and negative control respectively.</p><p><b>RESULTS</b>SBD containing serum significantly inhibited the proliferation of RA-FLS and OA-FLS, and decreased the secretion of IL-17 in RA-FLS. Its inhibition efficiency of SBD was equivalent to that of Leflunomide. No obvious inhibition on the secretion of IL-6 in RA-FLS was observed. It had no significant effect on the secretion of IL-17 and IL-6 in OA-FLS.</p><p><b>CONCLUSION</b>SBD could inhibit the proliferation of FLS and the secretion of IL-17 in RA-FLS, which might be one of its pharmacological mechanisms for treating RA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Arthritis, Rheumatoid
/
Synovial Membrane
/
Drugs, Chinese Herbal
/
Cells, Cultured
/
Interleukin-6
/
Rats, Sprague-Dawley
/
Bodily Secretions
/
Interleukin-17
/
Cell Biology
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2013
Type:
Article
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