Screening, cloning, and analyzing for hSNF5 binding proteins in human fetal brain / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 529-532, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-231893
ABSTRACT
<p><b>OBJECTIVE</b>To identify novel binding proteins of hSNF5, a subunit of chromatin remodeling complex in human fetal brain.</p><p><b>METHODS</b>The yeast two-hybrid system was used for this study. Positive cDNA clones were sequenced. Sequence homology and putative functional domains were analyzed and compared with databank.</p><p><b>RESULTS</b>Nine positive clones obtained were analyzed, among which the sequence of one clone was 97% homologous to the 3' mRNA of a hypothetical protein FLJ20643, while other four clones were related to protein coding sequences existed in the GenBank. The rest four clones were not in frame with any known protein coding sequence.</p><p><b>CONCLUSIONS</b>Clones encoding for hSNF5 binding protein exists in cDNA library of human brain. These proteins may recruit chromatin remodeling complex via hSNF5 to modulate the transcription of their target gene and the related cell functions.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
Transcription, Genetic
/
Brain
/
Chromosomal Proteins, Non-Histone
/
Trans-Activators
/
Cloning, Molecular
/
DNA, Complementary
/
Two-Hybrid System Techniques
/
Cell Biology
/
DNA-Binding Proteins
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS