BAY11-7082 and Lactacystein in CD154-induced NF-kappaB activation / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 488-491, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-231902
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibition mechanisms of BAY11-7082 (IkappaB-alpha phosphorylation inhibitor) and Lactacystein (proteosome inhibitor) in CD154-induced NF-kappaB activation.</p><p><b>METHODS</b>We used recombinant CD154 to stimulate EBV/LMP1 negative Ramos B cell and observed the effects of BAY11-7082 and Lactacystein in CD154-induced NF-kappaB luciferase activation, phosphorylation and degradation of IkappaB-alpha, phosphorylation of p65, and nuclear translocation of NF-kappaB subunits upon CD154 stimulation.</p><p><b>RESULTS</b>Both BAY11-7082 and Lactacystein abrogated CD154-induced NF-kappaB luciferase activation in Ramos cells. While CD154-induced phosphorylation of p65, phosphorylation and degradation of IkappaB-alpha, and nuclear translocation of p50, p65, and c-Rel were all blocked by BAY11-7082; Lactacystein only inhibited degradation of IkappaB-alpha and p65 nuclear translocation.</p><p><b>CONCLUSION</b>BAY11-7082 and Lactacystein inhibit CD154-induced NF-kappaB activation through different mechanisms.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Acetylcysteine
/
Sulfones
/
Tumor Cells, Cultured
/
Cysteine Proteinase Inhibitors
/
NF-kappa B
/
Burkitt Lymphoma
/
Apoptosis
/
CD40 Ligand
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article
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