Nano-ESI-MS/MS identification on differentiation-associated proteins in M1 mouse myeloid leukemia cells induced by IL-6 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 483-487, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-231903
ABSTRACT
<p><b>OBJECTIVE</b>To identify two differentiation-associated proteins induced by rhIL-6 in M1 mouse myeloid leukemia cells.</p><p><b>METHODS</b>Protein spots were excised from 2-D gels and digested in-gel with trypsin. The trypsin lysis products were first analyzed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) through peptide mass fingerprinting and then performed peptide sequencing by nano-electrospray ionization mass spectrometry/mass spectrometry (nano-ESI-MS/MS). The database search was finished with the Mascot search engine (http//www.matrixscience.co.uk) using the data processed through MaxEnt3 and MasSeq.</p><p><b>RESULTS</b>The two proteins were not revealed by peptide mass fingerprint using MALDI-TOF-MS, while they were respectively identified as Destrin and Putative protein after the sequence of their trypic peptides were obtained by the nano-ESI-MS/MS techniques.</p><p><b>CONCLUSION</b>Nano-ESI-MS/MS technique can successfully identify the two differentiation-associated proteins induced by rhIL-6 and has great advantage in protein analysis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Recombinant Proteins
/
Molecular Sequence Data
/
Leukemia, Myeloid, Acute
/
Cell Transformation, Neoplastic
/
Amino Acid Sequence
/
Interleukin-6
/
Apoptosis
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article
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