Your browser doesn't support javascript.
loading
Nano-ESI-MS/MS identification on differentiation-associated proteins in M1 mouse myeloid leukemia cells induced by IL-6 / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 483-487, 2004.
Article in Chinese | WPRIM | ID: wpr-231903
ABSTRACT
<p><b>OBJECTIVE</b>To identify two differentiation-associated proteins induced by rhIL-6 in M1 mouse myeloid leukemia cells.</p><p><b>METHODS</b>Protein spots were excised from 2-D gels and digested in-gel with trypsin. The trypsin lysis products were first analyzed by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) through peptide mass fingerprinting and then performed peptide sequencing by nano-electrospray ionization mass spectrometry/mass spectrometry (nano-ESI-MS/MS). The database search was finished with the Mascot search engine (http//www.matrixscience.co.uk) using the data processed through MaxEnt3 and MasSeq.</p><p><b>RESULTS</b>The two proteins were not revealed by peptide mass fingerprint using MALDI-TOF-MS, while they were respectively identified as Destrin and Putative protein after the sequence of their trypic peptides were obtained by the nano-ESI-MS/MS techniques.</p><p><b>CONCLUSION</b>Nano-ESI-MS/MS technique can successfully identify the two differentiation-associated proteins induced by rhIL-6 and has great advantage in protein analysis.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Recombinant Proteins / Molecular Sequence Data / Leukemia, Myeloid, Acute / Cell Transformation, Neoplastic / Amino Acid Sequence / Interleukin-6 / Apoptosis / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2004 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Recombinant Proteins / Molecular Sequence Data / Leukemia, Myeloid, Acute / Cell Transformation, Neoplastic / Amino Acid Sequence / Interleukin-6 / Apoptosis / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2004 Type: Article