Cloning of gdnf in the mouse testis and its expression in sertoli cells / 中华男科学杂志
National Journal of Andrology
;
(12): 975-978, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-232029
ABSTRACT
<p><b>OBJECTIVE</b>To clone the glial cell line-derived neurotrophic factor (GDNF) from the mouse testis, construct the eukaryotic expression vector and transfect this vector into Sertoli cells in order to use the gdnf-transfected Sertoli cells as the feeder layer to cultivate spermatogonial stem cells (SSCs).</p><p><b>METHODS</b>Total RNA was extracted from the testes of normal mature mice and gdnf was cloned and amplified using RT-PCR, inserted into the eukaryotic expression vector and transfected into sertoli cells (TM4 cell line). Immunofluorescence with anti-GDNF antibodies was performed at 40 h following the transfection.</p><p><b>RESULTS</b>gdnf cDNA was cloned successfully, and GDNF expressed after transfected into Sertoli cells.</p><p><b>CONCLUSION</b>This study provides a basis for culturing SSCs with gdnf-transfected Sertoli cells as the feeder layer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sertoli Cells
/
Testis
/
RNA
/
Transfection
/
Gene Expression
/
Cloning, Molecular
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Glial Cell Line-Derived Neurotrophic Factor
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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