Interruption failure of heptitis B virus vaccination in mother-to-infant transmission and heptitis B virus genotypes and preC/BCP mutations / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 331-333, 2007.
Article
in Zh
| WPRIM
| ID: wpr-232310
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of heptitis B virus (HBV) genotypes and precore(PreC)/basal core promoter(BCP) mutation with interruption failure of HBV vaccination in mother-to-infant transmission.</p><p><b>METHODS</b>A total number of 208 serum samples were collected from infants and mothers,including 16 infants who had become HBsAg-positive despite a complete and timely course of immunization and another 88 infants successfully protected from mother-to infant HBV transmission. HBV genotypes were determined by type-specific primers PCR method. PreC/BCP mutations were detected by direct sequencing of PCR products, and Clustal W 1.8 software was applied to analyzing the sequences.</p><p><b>RESULTS</b>Of 16 mothers who were having vaccine failure infants, 15 (93.8%) were HBeAg positive and infected with genotype C (15/15, 100%). Among 88 mothers of having children being protected by vaccine, 51 (58.0%) were HBeAg positive, with 45.1% (23/51) of genotype C. The proportion of genotype C in HBeAg mothers of infants with vaccine failure, was significantly higher than that of mothers with vaccine protected infants (chi2 = 14.3, P = 0.003). However, the frequencies of T1762/A1764 mutations had no significant differences between genotype C HBeAg positive mothers with vaccine failure or protected infants (33.3% and 13.3%, respectively, P = 0.4). No A1896 mutation was found in these two groups.</p><p><b>CONCLUSION</b>HBV genotype C might contribute to the immune failure of HBV vaccination in mother-to-infant transmission, while PreC/BCP mutation might not have correlation with it.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pregnancy Complications, Infectious
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Virology
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Hepatitis B virus
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Hepatitis B Vaccines
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Infectious Disease Transmission, Vertical
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Allergy and Immunology
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Genes, Viral
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Genetics
Limits:
Adult
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Female
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Humans
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Newborn
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Pregnancy
Language:
Zh
Journal:
Chinese Journal of Epidemiology
Year:
2007
Type:
Article